The Cyrano de Bergerac star was onboard a flight from Paris, France to Dublin, Ireland last month (Aug11) when he was caught short and needed to relieve himself while the aircraft was delayed on the runway.
Cabin crewmembers refused to allow him to use the toilets until after take-off, so Depardieu decided to pee into an empty bottle.
Urine splashed onto the floor, prompting the pilot to taxi back to the airport terminal so the plane could be cleaned - delaying the flight by a further two hours.
His friend and travel companion, actor Edouard Baer, claimed his pal had no choice but to use the bottle because he suffers from "prostate problems", although Depardieu himself has remained silent about the incident.
Now he has poked fun at the scandal by filming an 80-second video in character as the strong and overweight wild boar-eating Obelix, the sidekick of diminutive Gaulish warrior Asterix, portrayed by Baer.
In the clip, the pair is shown onboard a grounded plane when Depardieu loses his temper and throws a tantrum after being told he is not allowed to dine on his feast of boar until after take-off.
The actors are familiar with the popular French comic book characters - they are set to star in a big screen adaptation of the cartoon next year (12).
The 62-year-old star, who was travelling with fellow actor Baer, boarded a flight from Paris, France to Dublin, Ireland but was caught short when it was delayed on the runway.
He was told by the cabin crew that passengers were not allowed to visit the bathroom until after take-off - so he urinated in an empty bottle towards the rear of the plane. Urine splashed onto the floor, prompting the plane's pilot to taxi back to the terminal so the aircraft could be cleaned and the flight was delayed for a further two hours.
Baer has addressed the incident, insisting his friend is full of remorse for his actions but was unable to avoid the drama because of health problems.
In a statement, Baer says, "Gerard is very sorry for what happened. He wanted to go to the toilet but he was forbidden because we were about to take off, so he was forced to do it on the plane in front of everybody. I found the solution by passing him a mini Evian water bottle, but it was too small and overflowed.
"He has prostate problems and it was very worrying and humiliating for him. He was also stone-cold sober at the time. This is not the way he usually behaves."
The 62-year-old star boarded the City Jet flight from Paris to Dublin but was caught short when it was delayed on the runway.
He was told by the cabin crew that passengers were not allowed to visit the bathroom until after take-off - so he allegedly urinated in an empty bottle towards the rear of the plane.
Unfortunately a little urine splashed onto the floor, prompting the plane's pilot to taxi back to the terminal so the aircraft could be cleaned. The flight was delayed for a further two hours.
Reports suggest Depardieu was travelling with fellow French actor Edouard Baer, who has insisted the Green Card star had an "irrepressible need" to relieve himself and did so as discreetly as possible.
But an outraged female passenger on the flight has told France's Europe 1 radio network, "Mr Depardieu got up, calling out, 'I need to p**s, I need to p**s'.
"The air hostess replied, 'I am sorry, you'll have to wait 15 minutes until we are in the air, everyone is strapped in, the lavatories are shut, I can do nothing for you...' He could've waited a couple of minutes."
City Jet officials have confirmed that an incident took place onboard the plane but declined to name the individual involved.
A tweet from the airline company reads, "As you may have seen on the news, we are busy mopping the floor of one of our planes."
Depardieu has yet to comment about the incident.