Chastain, who is currently nominated for a Golden Globe for her turn in The Help, will hit the stage this autumn (12).
The actress will portray a shy young woman yearning for her wealthy father's acceptance in the show, which will be directed by Bengal Tiger at the Baghdad Zoo veteran Moises Kaufman.
Her role was most famously played on the big screen by Olivia de Havilland in 1949.
The play, which was written by Ruth and Augustus Goetz, premiered in New York in 1947. It last appeared on Broadway in 1995.