Actor Peter Facinelli, who plays vampire patriarch Carlisle Cullen in the film franchise, will lead a team of co-stars and crew against American footballers on the Saints roster in the 20 March (10) baseball battle.
Twilight stars Chaske Spencer, Alex Meraz and Tyson Houseman will also take part in the challenge, which will take place in New Orleans, Louisiana.
Proceeds from ticket sales will benefit the American Red Cross and Hope for Haiti Now. The Blood Center will host a blood drive during the event.
Facinelli admits he has become a big baseball fan after learning how to play the game for the first Twilight film.