Recent tabloid reports suggested the Dawson's Creek star was expecting a little brother or sister for the couple's three-year-old daughter, Suri.
Holmes was seen visiting a doctor's office, and photos of her surfaced with a bloated stomach wearing what appeared to be maternity clothes.
However her spokesperson insists the 31 year old isn't expecting another baby, telling People.com: "Katie is not pregnant."
Holmes and Cruise married in 2006.