This will be the second time that Knowles gives it a shot as an actress. She made her acting debut this year in the role of the MTV telefilm Carmen: A Hip Hopera.
Austin Powers 3 was written by Mike Myers and Michael McCullers and continues the adventures of superspy Austin Powers, his nemesis, Dr. Evil, and the villainous Fat Bastard, all played by Myers.
Last week, actor Michael Caine signed on to star as Austin Powers' father.
Although rumor has it that Destiny's Child will break up so that each member can pursue solo careers, their third album, Survivor, has sold more than 3 million units since its May release.
Last year the trio won a Grammy for R&B performance by duo or group for their hit "Say My Name."