Michele poses opposite The Creature From The Black Lagoon for one stylish black and white shot, while Agron conjures up memories of Attack of the 50ft Woman and Mays gives The Birds star Tippi Hedren a new look.
The trio's co-star Heather Morris also appears in the spread, recreating a classic Catherine Deneuve pose from Roman Polanski's Repulsion.
Michele admits she didn't need much persuasion to sign up for the shoot. She tells U.S. news show Entertainment Tonight, "I am the biggest horror film fanatic. It's one of my favourite things to do, crawl up at home and watch a scary movie."