Current Bond Daniel Craig was filmed in character leaving Buckingham Palace with the royal before climbing into a helicopter, and the pair appeared to parachute into London's Olympic Stadium during the extravaganza.
The high-profile tribute to the superspy came 50 years after the first movie, Dr. No, was released, and Moore - who played the iconic agent from 1973 to 1985 - is ecstatic the monarch took part.
He tells Britain's Daily Telegraph, "Wasn't she marvellous? What a tribute to Bond in its 50th year, to be recognised by the Queen in agreeing to do that.
"In that first scene, with Bond standing there when you saw the back of her head, I thought to myself, 'I wonder who they have got to double for the Queen?' and then when she turned around, I just thought, 'Wow.'"