The secret agent, currently portrayed by Daniel Craig in the 007 movies, beat the world's fastest man, Olympic gold medallist Usain Bolt, to the top of AskMen.com's survey, leaving the athlete trailing at two.
Explaining their choice for number one, website editors claim Ian Fleming's spy creation "has always fit right in with the times", adding: "James Bond has been influencing men for six decades, and he's not done yet."
Former U.S. President Bill Clinton placed third, while Family Guy creator Seth MacFarlane and The Dark Knight Rises star Joseph Gordon-Levitt round out the top five in fourth and fifth place, respectively.
Comedian Jimmy Kimmel landed in sixth, R&B singer/songwriter Frank Ocean at eight, and Hollywood hunk Ryan Gosling at 10.
Incumbent American leader Barack Obama takes number 11, while director Christopher Nolan (15), Liam Neeson (16), rap pals Jay-Z (22) and Kanye West (31), and actor Alec Baldwin (49) also make the list.