The Knocked Up star was among the famous faces who donated auction prizes for the Night of Too Many Stars benefit and he offered up the chance to share a toilet run with him.
Rogen's bizarre lot attracted plenty of bids and two lucky winners sealed the deal to share a bathroom with the actor, according to E! Online.
Other prizes offered up during the evening included a two-day visit to the set of hit TV show Breaking Bad, which sold for $22,000 (£13,750), and the chance to have a holiday card picture taken with actor Al Pacino, which went for $25,000 (£15,625).
Former Saturday Night Live co-stars Tina Fey and Amy Poehler offered up their company to the highest bidder, and two women paid $36,000 (£22,500) to be the comediennes' friends for the night.
The event at Manhattan's Beacon Theater also included performances by Carly Rae Jepsen, who sang her hit track Call Me Maybe with actor Harvey Keitel, and Katy Perry, who belted out her song Firework with a young autistic girl accompanying her on piano.
The bash raised funds for a number of autism education and family service programs across the U.S.