The former Baywatch star will dress up as Captain Hook from Peter Pan for the recording and he has pledged to donate his winnings to Pengeulan Primary School in Wales, a facility he visited in September (12).
He says, "I just hope I can win some big bucks for them. One minute I'm terrified, the next I can't wait to jump in to the seat. I've been gearing up and I'm ready to face the million pound question."
The Who Wants To Be A Millionaire? Pantomime Special airs in Britain on 9 December (12).
Hasselhoff will play Captain Hook in a festive pantomime production of Peter Pan in Manchester, England starting from next month (Dec12).