The 1964 DB5 has been dubbed "the world's most famous car" by auction house RM Auctions and the experts expect the motor to fetch over $5 million (£3.3 million).
The car is the last remaining model made for Connery to drive as 007 in the films Goldfinger and Thunderball.
The motor comes complete with machine guns, a bullet-proof shield, revolving number plates, oil slick sprayer and smoke screen, along with other gadgets.
The car is being sold by U.S. radio broadcaster Jerry Lee - the motor's only ex-factory owner.
Lee plans to donate the proceeds to charity.