Knowles and Larter's headbutting brawl in 2009 suspense thriller Obsessed won the pair the top prize in the Best Fight category, announced during the red carpet pre-show in Los Angeles.
Tom Cruise then kicked off the ceremony, reviving his foul-mouthed, bad-tempered Tropic Thunder character Les Grossman for a hilarious skit featuring Twilight stars Taylor Lautner and Robert Pattinson.
Will Smith's 11-year-old son, The Karate Kid star Jaden Smith, also appeared, showing off his fighting skills in a showdown against Cruise's character.
Twilight star Kristen Stewart picked up the first on-stage honour for Best Female Performance for her role as Bella Swan in the vampire franchise, beating out Sandra Bullock (The Blind Side), Zoe Saldana (Avatar), Emma Watson (Harry Potter) and Amanda Seyfried (Dear John).
Accepting the award, Stewart kept her speech short, telling the crowd, "Thank you so much this is awesome. I guess Twilight is really awesome and I agree. I want to say hi to Chris (Weitz) because I haven't talked to him in a while. He directed the movie (Twilight sequel New Moon) and I really loved him and how cool is this, right? I'm going to peace out. Thank you so much."