Activists from the AIDS Health Foundation claim the publisher should encourage adult porn stars to use condoms.
As part of the grievance, filed with the Division of Occupational Safety and Health in Los Angeles, campaigners delivered 100 of Flynt's DVDs in a bid to demonstrate that only one scene out of all the movies showed a star using protection.
The complaint claims the movies "clearly demonstrate workplace activities highly likely to spread bloodborne pathogens in the workplace", reports the Associated Press.
But Larry Flynt Productions President, Michael Klein, insists porn viewers don't want to see condoms in the films, saying in a statement, "We won't budge when it comes to condomless productions. That's what the consumer wants, and we deliver it."
The complaint is now being looked into.