Stuart passed away on Sunday (26Sep10) at her Los Angeles home, reports The Washington Post.
Her daughter, writer Sylvia Thompson, tells the newspaper Stuart was diagnosed with lung cancer several years ago and had also previously battled breast cancer.
The actress began working in Hollywood in the 1930s and starred in over 40 films during her career, including The Invisible Man, Here Comes the Navy and Poor Little Rich Girl.
After a nearly a decade away from the spotlight, she returned to the big screen in 1997, as the 101-year-old version of Kate Winslet's character Rose in James Cameron's Titanic.
She became the the oldest actress ever to be nominated for an Oscar for her supporting role in the film.
Stuart published her autobiography, I Just Kept Hoping, in 1999, and received a star on the Hollywood Walk of Fame in 2000.
She last appeared on the big screen in Wim Wenders' Land of Plenty in 2004.
The star was honoured by the Screen Actors Guild for her years of service in June (10), weeks before celebrating her centenary on 4 July (10).
In addition to her daughter, Stuart is survived by four grandchildren and 12 great-grandchildren.