They will share the stage with Glee stars Lea Michele and Matthew Morrison, as well as Lost actor Jorge Garcia, Nip/Tuck's Julian McMahon and Ricki Lake for The Rocky Horror Picture Show Tribute later this month (Oct10).
The show, which also features a costume ball for guests, will be held to raise money for the Painted Turtle, a charity set up by late actor Paul Newman to help children with chronic and life-threatening illnesses.
The musical will feature choreography from famed dance guru Kenny Ortega, while Lou Adler, who executive-produced the 1975 big screen version of the Rocky Horror stage show, is taking charge of the production.
He tells Variety, "It is terrific to have such a diverse cast of actors in support of a great cause - The Painted Turtle. Each of these individuals will bring a unique performance to the tribute and make for an incredibly memorable evening."
The show wil take place on 28 October (10) at the Wiltern Theater in L.A.