The star-studded cast took to the stage at the city's Wiltern Theatre to stage a production of the classic musical in aid of Paul Newman's Painted Turtle charity.
Nip/Tuck's Julian McMahon thrilled fans by donning a corset and suspenders to play Dr. Frank-N-Furter, while Glee stars Michele and Morrison took on the roles of stranded lovers Janet Weiss and Brad Majors.
The show also saw star turns from Jack Nicholson, Nicole Scherzinger, Billy Idol, Danny Devito, Ricky Lake, Rachel Evan Wood, Jason Segel and Lost's Jorge Garcia.
Speaking before the event, producer Lou Adler, who worked on the original film, admitted McMahon wasn't his first choice to play Frank-N-Furter - he wanted Cher to tackle the role.
He says, "I've always envisioned Cher as Frank-N-Furter. But she's working in Las Vegas."