Married... With Children actor O'Neill came under fire after allegedly questioning Lynch's Emmy Award win for her role in Glee, hailing Vergara as a more worthy recipient.
He later insisted his quotes were taken out of context and phoned Lynch to clear up his comments.
Vergara has now laughed off any shortlived feud between the two casts by dressing up as Lynch's tough character Sue Sylvester in a new video for FunnyorDie.com.
In the clip, she plays a Lynch superfan, telling her fellow video stars, including Glee's Heather Morris, “Comparing Jane to the rest of the cast is like comparing the Great Barrier Reef with a piece of dog s**t. No offence, Heather.”
Morris then raps about the success of the show while her Glee co-stars Matthew Morrison, Cory Monteith, Harry Shum, Jr., and Naya Rivera all appear in the hip-hop inspired promo.