The ABBA stage show became a box office phenomenon when it was transformed into a 2008 film, overtaking Grease as the world's top-earning movie musical after pulling in a mammoth $600 million (£400 million) in global ticket sales.
Following the film's success, Seyfried admitted she spread rumours of a sequel around Hollywood because she was desperate to return to her role as lovestruck Sophie.
But now the actress has conceded she will never play the part again - as film bosses would struggle to put together a new installment.
She tells Britain's Marie Claire magazine, "F**k no! That's never gonna happen. Like, what would they do? What would they write about? How would they get all the actors back?"