The Clueless star played fashion boutique owner Val Stokes in the first series of the VH1 comedy, which is filmed in Atlanta, Georgia.
But Dash has decided to leave the show before season two begins shooting to spend more time with her family, who live in Los Angeles.
She tells GlobalGrind.com, "I truly enjoyed playing Val on Single Ladies, but I have decided to leave the show. I have to be back in L.A. with my children right now and the Single Ladies shooting location makes that impossible.
"I wish VH1 the best of luck with the show and in maintaining the strong fan base we developed in season one."
A spokesperson for the network adds, "VH1 respects Stacey's decision and her commitment to what she feels is best for her family. We thank her for all of her hard work in making Single Ladies a success right out of the gate, and we wish her nothing but the best in her future plans."