The couple spent its sixth wedding anniversary on 24 September (11) apart and the usually-chatty Moore recently stunned reporters at the premiere of her latest film project Five when she refused to answer questions about her husband on the red carpet.
New unconfirmed reports of Kutcher's infidelity have since emerged, prompting gossips to speculate further about the split and examine Moore's cryptic tweets in recent weeks.
On 23 September (11), Moore quoted Greek philosopher Epictetus, and wrote, "When we are offended at any man's fault, turn to yourself & study your own failings. Then you will forget your anger." But now the note has taken on a new meaning, suggesting she and Kutcher may have fallen out.
Kutcher and Moore have yet to directly address the break-up claims, but now the Two and a Half Men star appears to be playing down the rumours with a mystery tweet of his own.
On Thursday morning (29Sep11), Kutcher wrote "now playing", followed by a link to his Spotify music account to the hip-hop classic, suggesting the tabloids have his marriage troubles all wrong.
Kutcher later added, ""When you ASSUME to know that which you know nothing of you make an ASS out of U and ME."