The Supernatural star has asked fans to keep his beloved Harley in their thoughts after the pooch lost its battle with cancer.
Addressing his followers on Twitter.com, Padalecki writes, "Can I have 195000 moments of silence for a great dog who lost his battle with cancer?... R. I. P. Harley. We're better for having known you.
"Thank y'all so much for your support during this very difficult time. It's meant the world to us. I'm so truly grateful for your kindness."