Biel, who dressed in a catsuit to portray the comic book villain for a Halloween party scene in 2007 comedy I Now Pronounce You Chuck & Larry, admits she jumped at the chance to audition for a leading role The Dark Knight Rises, but she was left licking her wounds when director Christopher Nolan handed the role to Hathaway.
She tells Elle magazine, "(That was) another one that got away. I was gutted. I mean, to work with Christopher Nolan in that kind of a role..."
Biel then set her sights on landing the part of prostitute Fantine in filmmaker Tom Hooper's retelling of the classic Victor Hugo story, but admits nerves got the better of her as she tried out for the job.
She says, "I felt terrified when I auditioned for Les Miserables, the movie that Tom Hooper is doing. I was terrified to sing in front of him. I auditioned for Fantine and I sang I Dreamed a Dream. I didn't get it. And I would have loved it."
Hathaway signed on for Les Miserables last month (Oct11), joining Hugh Jackman, Russell Crowe and Helena Bonham Carter in the ambitious new film.
In the same Elle interview, Biel revealed she also missed out on the romantic lead in The Notebook to Rachel McAdams.