The bloodsucker film was named best movie drama in the ceremony at Universal Studios' Gibson Amphitheatre, with its stars Robert Pattinson and Kristen Stewart taking home best actor trophies.
The hit 2008 picture also secured wins in a string of off-beat categories including best romance and best liplock, while Cam Giganget was named best villain and Taylor Lautner lauded as the year's fresh face male.
Pattinson was also named choice male hottie alongside choice female hottie Megan Fox.
Other big winners in the acting categories were Hugh Jackman who picked up two prizes for his comic book blockbuster X-Men Origins: Wolverine, Zac Efron who landed two honours for his role in 17 Again and a further award for his turn in High School Musical 3: Senior Year.
Teen drama Gossip Girl ruled the TV section, picking up four awards, including best drama show and best actor awards for Leighton Meester and Chace Crawford. Ed Westwick was named best TV villain.
Miley Cyrus landed four surfboard trophies in the acting categories - two for her big screen outing in Hannah Montana: The Movie and two for her Hannah Montana TV show. Her father Billy Ray Cyrus was named TV's top parental unit for his part in the teen franchise and her co-star Emily Osment was named best TV sidekick.
Cyrus also led the way in the music categories with her hit track The Climb being named single of the year and her Jonas Brothers collaboration Before the Storm winning best summer song.
Taylor Swift was named best female artist and handed the best female album award for her hit record Fearless while Jason Mraz landed the male equivalent in both categories, winning best male album for We Sing, We Dance, We Steal Things.
Other music stars to pick up honours included Kanye West (rap artist), Beyonce (R&B artist) and Paramore (rock group).
The Jonas Brothers, who hosted the show and performed their track Much Better, won five surfboards, including male red-carpet icons and breakout TV show (Jonas).
The night also saw performances from the Black Eyed Peas, Sean Kingston and Miley Cyrus, who went on to present Britney Spears with the coveted Ultimate Choice award, telling the crowd, "I'm a big fan of hers!.
The list of main winners is as follows.
Movie Action Adventure: X-Men Origins: Wolverin.
Movie Drama: Twiligh.
Movie Bromantic Comedy: Marley & M.
Movie Comedy: Night at the Museum: Battle of the Smithsonia.
Movie Chick Flick: 27 Dresse.
Movie Horror/Thriller: Friday the 13t.
Movie Music/Dance: High School Musical 3: Senior Yea.
Movie Hissy Fit: Miley Cyrus, Hannah Montana: The Movi.
Movie Actor Drama: Robert Pattinson, Twiligh.
Movie Actress Drama: Kristen Stewart, Twiligh.
Movie Actor Action Adventure: Hugh Jackman, X-Men Origins: Wolverin.
Movie Actress Action Adventure: Jordana Brewster, Fast & Furiou.
Movie Actor Comedy: Zac Efron, 17 Agai.
Movie Actress Comedy: Anne Hathaway, Bride War.
Movie Actress Music/Dance: Miley Cyrus, Hannah Montana: The Movi.
Movie Actor Music/Dance: Zac Efron, High School Musical 3: Senior Yea.
TV Show Drama: Gossip Gir.
TV Show Action Adventure: Heroe.
TV Show Comedy: Hannah Montan.
TV Actor Drama: Chace Crawford, Gossip Gir.
TV Actress Drama: Leighton Meester, Gossip Gir.
TV Actor Comedy: Jonas Brothers, Jona.
TV Actress Comedy: Miley Cyrus, Hannah Montan.
TV Breakout Show: Jona.
TV Villain: Ed Westwick, Gossip Gir.
TV Parental Unit: Billy Ray Cyrus, Hannah Montan.
Music Single: Miley Cyrus, The Clim.
Choice Music Hook-Up: Lady Ga Ga and Colby O'Donis, Just Danc.
Music Male Artist: Jason Mra.
Music Female Artist: Taylor Swif.
Music Rap Artist: Kanye Wes.
Music R&B Artist: Beyonc.
Music Rock Group: Paramor.
Music Album Male Artist: Jason Mraz, We Sing, We Dance, We Steal Thing.
Music Album Female Artist: Taylor Swift, Fearles.
Choice Award: Britney Spears.