The Do The Right Thing director accuses Perry of "coonery and buffoonery" in his films - comments the younger filmmaker has taken exception to.
In a taped interview for U.S. news show 60 Minutes, Perry says, "That p**ses me off. It really does - because it's so insulting.
"It's attitudes like that that make Hollywood think that these people (his characters) do not exist and that's why there's no material speaking to them, speaking to us. I would love to read that to my fan base."
It's not the first time Lee has picked a fight with a big name movie icon - he attacked Quentin Tarantino over the use of the 'N' word in his films and also took aim at Clint Eastwood for failing to include African-Americans in his war movies Flags of Our Fathers and Letters From Iwo Jima.