The public cast more than 18 million votes online to select the nominees for next year's (10) ceremony, and will also choose the winners in 35 categories.
The Twilight Saga will compete against Harry Potter and the Half-Blood Prince and Star Trek in the favourite movie and favourite film franchise categories.
Stars Kristen Stewart, Robert Pattinson and Taylor Lautner have earned nods for best on-screen team, joining fellow nominees Daniel Radcliffe, Rupert Grint and Emma Watson (Harry Potter and the Half-Blood Prince), Sandra Bullock and Ryan Reynolds (The Proposal), Shia LaBeouf and Megan Fox (Transformers: Revenge of the Fallen) and Hugh Jackman, Liev Schreiber, Ryan Reynolds, will.i.am, Dominic Monaghan and Daniel Henney (X-Men Origins: Wolverine).
Pattinson and Stewart were also nominated individually for favourite movie actress and actor, and will face-off against A-listers Brad Pitt, Hugh Jackman, Johnny Depp, Ryan Reynolds, Anne Hathaway, Drew Barrymore, Jennifer Aniston and Sandra Bullock in their respective categories.
Lautner is also up for breakout movie actor alongside Chris Pine, Joseph Gordon-Levitt, Sam Worthington and Zachary Quinto.
Meanwhile, True Blood was nominated for favourite TV obsession and favourite sci-fi/fantasy TV show, where it will battle for the title against The Vampire Diaries, Heroes, Lost and Supernatural.
The show's star Anna Paquin will fight it out with Blake Lively, Jennifer Love Hewitt, Katherine Heigl and Mariska Hargitay for favourite TV drama actress - while Hugh Laurie, Kiefer Sutherland, Mark Harmon, Matthew Fox and Patrick Dempsey compete for the title of favourite TV drama actor.
Musically, newcomers Adam Lambert, Demi Lovato, Kris Allen, Lady Gaga and reality TV star Susan Boyle will battle for favourite breakout music artist.
Eminem, Jason Mraz, John Mayer, Keith Urban and Tim McGraw will go head-to-head for the favourite male artist accolade, while their female counterparts include: Beyonce, Britney Spears, Carrie Underwood, Pink and Taylor Swift.
Queen Latifah will host the People's Choice Awards at Los Angeles' Nokia Theatre on 6 January, 2010.