The footage of Irwin's last moments on Sept. 4 last year was used in a police report and coroner's investigation into his death, but the original copy has now been returned to Irwin's widow, Terri, and all other copies have been destroyed.
The tape, reportedly showing Irwin snorkeling above the stingray before it whipped its tail into him, had reportedly been the subject of a million-dollar bidding war from several Web sites.
Irwin's manager, John Stainton, said, "Anything to do with the day that he died, that film is not available."
Michael Barnes, the Australian coroner investigating the case, insisted he'd taken "all possible steps to ensure something of such a personal and tragic nature did not fall into the wrong hands."
Irwin's last program, Ocean's Deadliest, featuring footage shot in the week before Irwin's death, will be shown for the first time in the U.S. on the Discovery Channel on Jan. 21.
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